ELABORAÇÃO DE UM SOFTWARE PARA REALIZAR ESTIMAÇÃO DE PARÂMETROS APLICADOS À CINÉTICA ENZIMÁTICA

Abstract

Parameter estimation for mathematical models related to enzyme kinetics, as reported in the literature, has been usually inappropriate, due to disregard of suitable statistical analysis. The most common model used is the Michaelis-Menten one. Such model often has been rewritten on linear forms for its parameter estimation. It simplifies the estimation, however, it fails to give due consideration to statistical assumptions. The present work aimed to develop software, supported by the Fortran 90 programming language, capable to do appropriate estimation of kinetic model parameters, through linear regression and non-linear form, according to the model preferred by the user. To achieve this, the necessary adaptations were made on ESTIMA. It is of great importance good parameter estimation, especially when it is intended to project a process in bigger scales. Therefore, this software should be a very useful tool in academic and industrial scenes.

Index Terms - Enzyme kinetics, Fortran 90, Michaelis-Menten, parameter estimation.