DESENVOLVIMENTO DE BIOSSENSSOR ENZIMÁTICO A BASE DE EXTRATO DE SEMENTE DE PINHÃO-MANSO (Jatropha curcas L.) PARA DETECÇÃO DE COMPOSTOS FENÓLICOS

Abstract

Within the framework of environmental monitoring, the development of enzyme biosensors for detection of phenolic compounds has been focused by several researches. Phenolic compounds can be associated to pollution, depending on its nature and its concentration. The seeds of the species Jatropha curcas L., also known as physic nut, have been studied as an alternative source of biodiesel, however, the filter cake produced as by-product of oil extraction has a biochemical potential still little explored. In this work, it was obtained enzymatically active extracts from the filter cake made of physic nut seeds. Biosensors were modified from 1 up to 25% of biological element percentages. Scans were carried out using differential pulse voltammetry with variations on amplitude and scan speed. The biosensor presented activity in the presence of phenolic compounds and electroactivity change as a function of contact time with substrate.

Index Terms - electrochemistry, biosensor, water.