DESENVOLVIMENTO E VALIDAÇÃO DE METODOLOGIA ANALÃTICA PARA DETERMINAÇÃO DE FÃRMACOS EM AMOSTRAS DE ÃGUA, SUPERFICIAL E TRATADA, UTILIZANDO A CROMATOGRAFIA LÃQUIDA DE ULTRA PERFORMANCE ACOPLADA A ESPECTROMETRIA DE MASSAS TANDEM (UPLC-MS/MS)

Abstract

The objective of this study was to develop and validade analytical methods for residues of drugs (aspirin, sodium diclofenac, paracetamol, ibuprofen and fenoprofen) in water using ultra performance liquida chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). We used two different mobile phases. For the drugs paracetamol and diclofenac was used as mobile phase water:methanol (1:1, v/v) with addition of formic acid in positive electrospray ionization mode. For drugs acetyl salicylic acid, ibuprofen and fenoprofen was used mobile phase water:methanol (1:1, v/v) with addition of ammonium acetate and negative electrospray ionization mode. The performance of the method was evaluated on the following parameters: specificity and selectivity, working range, lineariry, limit of detection and limit of quantification, accuracy, ruggedness and uncertainty measurement.